Ion trap-orbitrap hybrid mass spectrometer with MALDI and ESI

University of Salzburg

Salzburg | Website

Large equipment

Short Description

High-performance linear ion trap-orbitrap hybrid mass spectrometer with electrospray ionization, nanoelectrospray ionization and matrix-assisted laser desorption / ionization, sample inlet through direct infusion with syringe pump, coupling with liquid chromatography or via stainless steel targets, control and evaluation via Xcalibur, computer, license for MASCOT

Contact Person

Prof. Dr. Christian Huber

Research Services

HPLC-MS measurements

Methods & Expertise for Research Infrastructure

The device is used for highly sensitive detection and identification of biomolecules based on the accurate mass or fragmentation spectra. By means of such measurements, for example, expression profiles of proteins or degradation products of drugs can be analyzed.

Allocation to Core Facility

Proteomics

Prof. Dr. Christian Huber
Fachbereich Molekulare Biologie und Schwerpunkt ACBN
0043 662 8044 5738
c.huber@sbg.ac.at
http://www.uni-salzburg.at/index.php?id=77&MP=77-44794
Please contact the research service of the University of Salzburg (Technologie@sbg.ac.at) or the responsible contact person for this section mentioned in the contact field.
Predict-IV – Profiling the toxicity of new drugs; a non animal-based approach integrating toxicodynamics and biokinetics
2008-2013
Wolfgang Dekant, Koordinator
Commission of the European Communities
http://www.predict-iv.toxi.uni-wuerzburg.de/

MARINA: Managing the risks of Nanoparticles
2008-2013
Lang Tran, Koordinator
Commission of the European Communities
http://www.marina-fp7.eu/

Doktoratskolleg Immunity in Cancer and Allery; W 1213
2008-2017
Josef Thalhammer, Koordinator
Fonds zur Förderung der wissenschaftlichen Forschung
http://www.uni-salzburg.at/phd-program-ica

Christian Doppler Laboratory for Innovative Tools for the Characterization of Biosimilars
2013-2020
Christian Huber, Laborleiter
Christian Doppler Forschungsgesellschaft
http://www.uni-salzburg.at/index.php?id=63471&MP=200731-200747%2C77-44794&L=1
Application of integrated transcriptomic, proteomic and metabolomic profiling for the delineation of mechanisms of drug induced cell stress
2013
Wilmes A., Limonciel,A., Aschauer L., Moenks,K., Bielow C., Leonard M.O., Hamon J., Carpi D., Ruzek S., Handler A., Schmal O., Herrgen K., Bellwon P., Burek C., Truisi G.L., Hewitt P., Di Consiglio E., Testai E., Blaauboer B.J., Guillou C., Huber C.G., Lukas A., Pfaller W., Mueller S.O., Bois F.Y., Dekant W., Jennings P.
Journal of Proteomics
DOI: dx.doi.org/10.1016/j.jprot.2012.11.022

Quantitative HPLC-MS Analysis of Nucleotide Sugars in Plant Cells following Off-Line SPE Sample Preparation
2014
Behmüller R., Forstenlehner I.C., Tenhaken R., Huber C.G.
Analytical and Bioanalytical Chemistry
DOI: 10.1007/s00216-014-7746-3

Rapid and Comprehensive Impurity Profiling of Synthetic Thyroxine by Ultrahigh-Performance Liquid Chromatography−High-Resolution Mass Spectrometry
2013
Neu V., Bielow C., Gostomski I., Wintringer R., Braun R., Reinert K., Schneider P., Stuppner H., Huber C.G.
Analytical Chemistry
DOI: dx.doi.org/10.1021/ac303722j

Nephron Toxicity Profiling via Untargeted Metabolome Analysis Employing a High-Performance Liquid Chromatography-Mass Spectrometry-Based Experimental and Computational Pipeline
2015
Ranninger C., Rurik M., Limonciel A., Ruzek S., Reischl R., Wilmes A., Jennings P., Dekant W., Kohlbacher O., Huber C.G.
Journal of Biological Chemistry
http://www.jbc.org/cgi/doi/10.1074/jbc.M115.644146

Site-Specific Characterization and Absolute Quantification of Pegfilgrastim Oxidation by Top-Down High-Performance Liquid Chromatography-Mass Spectrometry
2015
Forstenlehner I., Holzmann J., Toll H., Huber C.G.
Analytical Chemistry
DOI: 10.1021/acs.analchem.5b02029

Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components. Journal of Nanobiotechnology
2015
Boyles M.S.P., Kristl T., Andosch A., Zimmermann M., Tran N., Casals E., Himly M., Puntes V., Huber C.G., Lütz-Meindl U., Duschl A.
Journal of Proteomics
DOI: 10.1186/s12951-015-0146-9