Monolith NT115 BLUE/RED

Vienna Biocenter Core Facilities GmbH (VBCF)

Wien | Website

Großgerät

Kurzbeschreibung

Microscale Thermophoresis (MST) is a new method developed to measure biomolecular interactions. The Monolith NT.115 Instrument uses fluorescence detection with either a blue (470/520) or red (625/680) filter. Labeling of a biomolecule with a fluorescent dye will enable measurement of the dissocation constant between the molecule and a variety of ligands (protein-protein, protein-nucleic acid, nucleic acid-nucleic acid, etc).
http://www.nanotemper-technologies.com/products/monolith-series/monolith-nt115-series/

Ansprechperson

Peggy Stolt-Bergner

Research Services

The ProTech facility will either provide training and help with the experiment design so that you can book the instrument, or we can carry out the whole experiment for you.

Methoden & Expertise zur Forschungsinfrastruktur

Microscale Thermophoresis is a new method to cost efficiently measure molecular interactions, especially interactions of biomolecules.

General protein - ligand interactions can be measured (protein - protein, protein - DNA/RNA, protein - small molecule/ion).

The NT.115 BLUE/RED uses fluorescence as a read-out with either the BLUE filter (exc 450 em 520) or the RED filter (exc 620 em 680) therefore one of the binding partners needs to be fluorescently labeled.

Affinities as well as binding energies and stoichiometry of the molecular interaction can be determined.

Zuordnung zur Core Facility

Protein Technologies

Peggy Stolt-Bergner
Vienna Biocenter Core Facilities (VBCF)
+43 1 7962324 7070
peggy.stolt@vbcf.ac.at
http://www.vbcf.ac.at/protech
The Vienna Biocenter Core Facilities (VBCF) is a research institute providing access to state of the art infrastructure and advanced research services on a fee-for-service basis. Additionally, VBCF core facilities are open to all forms of scientific cooperation. Information on general conditions of cooperation and usage fees is provided upon request.
please refer to the Publications section of our website: http://www.vbcf.ac.at/facilities/protein-technologies/